A. CleanAmp™ dNTPs can be used with a broad selection of DNA polymerases including Taq, Pfu, Pfu (exo-), Dynazyme™, Deep VentR™, Tth and Tfi. When using CleanAmp™ dNTPs with these polymerases, all were able to produce the desired amplicon. Learn More…
Posted: October 7th, 2011 under CleanAmp, Hot Start PCR, PCR.
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A. No, CleanAmp™ dNTPs are reverted back to the corresponding standard dNTP prior to being incorporated into the amplicon. The 3’-hydroxyl modification blocks enzyme incorporation until it is removed during heat activation. After heat activation, the enzyme efficiently incorporates the corresponding standard dNTP.
Posted: September 27th, 2011 under CleanAmp, Nucleotides.
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A. A phosphoramidite is the actual building block we use to chemically synthesize oligonucleotides. It usually consists of a protected nucleoside with a 3’ phosphitylating reagent, where phosphorus is in the trivalent state and protected with a stable amine and ester. Learn More…
Posted: September 20th, 2011 under Oligonucleotides, phosphoramidite, Synthesis.
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Q. Can I buy linker reagents directly from TriLink?
A. Yes, click here to view the reagents still sold through TriLink. All of our other DNA synthesis reagents are now sold through Glen Research.
Posted: September 12th, 2011 under DNA, Linker, Synthesis.
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Q. Can TriLink label nucleotides with a DNA intercalating dye (e.g. Thiazole Orange)?
A. Yes, we are able to label nucleotides with a DNA intercalating dye. Using TriLink’s custom synthesis service, we can conjugate your dye of choice to dNTPs containing the appropriate linker. Read More…
Posted: August 29th, 2011 under DNA, Dye, Nucleotides.
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Q. Does TriLink provide LNA modified oligonucleotides?
A. Yes, we are able to synthesize LNA modified oligonucleotides, however there are a few stipulations. Since this material is under license by Exiqon we require that our customers obtain a license from Exiqon, purchase the LNA amidites and have the material shipped to our facility for synthesis.
Posted: August 22nd, 2011 under LNA, Oligonucleotides, Synthesis.
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Q. Why does the price of trimer modified oligonucleotides vary?
A. Trimer modification requires a highly custom synthesis protocol in which multiple additional factors are taken into account. Each trimer mix job is individually analyzed in order to provide the most cost effective pricing in terms of synthesis cost and trimer amidite usage. Read More…
Posted: August 11th, 2011 under Oligonucleotides, Synthesis, Trimer.
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A. CleanAmp™ dNTPs are able to decrease the formation of primer dimer products while increasing the specificity of the desired product better than unmodified dNTPs. Use of CleanAmp™ dNTPs also reduces data fluctuation, giving a more consistent PCR result.
Posted: July 11th, 2011 under CleanAmp, PCR.
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Q. Is it be possible to synthesize an RNA oligo of 60-90 nucleotides long? In addition, is it be possible to label some inner selected nucleotides as we desire? - Francisco Hernandez-Torres, University of Jaen
A. Yes, we can synthesize a 60-90mer RNA oligonucleotide. We have successfully made up to a 110mer unmodified RNA oligonucleotide and continue to try longer sequences as our customer’s request them. Yes, internal modifications can be incorporated. Read More…
Posted: July 5th, 2011 under Oligonucleotides, RNA, Synthesis.
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Dengue fever is believed to infect 50 to 100 million people worldwide annually. Rate of infection has dramatically increased by 30 fold over the past 50 years. Although there are a number of antiviral therapies on the market, there are no antidengue medications available. Read Article
Posted: June 28th, 2011 under Biotin, Modified Nucleoside Triphosphate.
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