CleanAmp™ 7-deaza-dGTP and CleanAmp™ 7-deaza-dGTP Mix function well on their own for most sequences with <80% GC-content. However, should you have a problematic target or one that is >80% GC-content, addition of any one of these additives may increase specificity. We have found 0.5M Betaine or 10% Glycerol give the best results.
Preliminary results show CleanAmp™ 7-deaza-dGTP Mix functions with a variety of enzymes. However, we recommend Invitrogen™ Taq DNA polymerase for best results. For questions regarding your specific enzyme, please contact us.
CleanAmp™ 7-deaza-dGTP Mix contains the CleanAmp™ modified nucleoside triphosphates of dA, dC, (dG:7-Deaza-dG) and dT at 10 mM. The dG mix is a 1:3 ratio of CleanAmp™ dGTP:CleanAmp™ 7-deaza-dGTP.
We are proud to introduce the latest addition to the CleanAmp™ Product Line, CleanAmp™ 7-deaza-dGTP for GC-rich target amplification. When DNA targets high in GC content are amplified, PCR product formation can often be compromised by inadequate strand separation and the propensity for complex secondary structure formation. The inability of the DNA polymerase to negotiate through regions of strong secondary structure, such as hairpins, often results in the formation of truncated PCR products. In addition, mis-priming amplification products are prevalent when primers are high in GC content. Despite the inherent challenges, detection of GC-rich sequences is becoming increasingly important for the molecular diagnosis of inherited diseases. Read More…