We are able to synthesize dinucleotides and trinucleotides. Please contact firstname.lastname@example.org for information or to obtain a quotation.
We are able to synthesize dinucleotides and trinucleotides. Please contact email@example.com for information or to obtain a quotation.
The ChromaLink™ One-Shot Biotin Kit (B-9007) biotinylates 100 µg of antibody at 1 mg/mL. We also have an additional kit, the ChromaLink™ Biotin Labeling Kit (B-9007) which is more flexible in format and can label up to 5 reactions of between 25 µg to 1 mg per 100 µL reaction. The reaction conditions were specifically designed to match the concentrations and quantities of most commercially available antibodies.
Yes. All proteins and antibodies may be labeled with ChromaLink™ Biotin or DIG labels. These products label primary amino groups on lysines found in proteins. A cysteine-labeling ChromaLink™ biotin reagent is also available (Cat. No. B-1012).
Dear Lee Hye,
Thank you for your question. We lyophilize our oligonucleotides in a lyophilizer or speed vac. Please let us know if you have any additional questions.
This is something you should determine experimentally for your protein of interest and your library.Our biologists have provided a few publications which describes a RNA and DNA selection protocol which may have additional information
Product Specialist I
Thank you for your email. While we recommend sticking to 2 uL of RNA input when possible, we found that we can still obtain good library prep results with up to 10 uL of RNA (without changing any other volumes in the 3′ Adapter Ligation Reaction). We recommend that you carry the entire volume of this reaction (10 uL + excess) to each of the next steps. This means that each subsequent reaction will now contain larger volumes than outlined in the manual.
Please let me know if you have any additional questions.
We ship our triphosphates on dry ice as a precaution. They are stable at room temperature, and even higher temperatures, for short periods of time. The chance of degradation during transit is very low. We recommend analyzing the material by AX-HPLC and then testing it in your application, provided the cost of the assay is not prohibitive. Contact us to request our analysis method.
All of our stocked items are non-biological, non-hazardous research reagents and do not pose a ship hazard. For custom synthesized compounds please contact us to determine if there is any issue or concern.
Yes. Please contact us to request a proforma invoice.
No, you will not be billed/charged until your order is shipped. We accept payment by purchase order, wire transfer and credit card (VISA, MasterCard and American Express). Terms are net 30. Please contact us for more information.
Our license to the technology requires that we pay a royalty for molecular beacons sold to institutions that do not have a license from The Public Health Research Institute of New York (PHRI). If you have a license, we do not have to pay a royalty to PHRI on your beacon. When you place your first order for a molecular beacon, we will ask if you have a license from PHRI.
The batch record for your product is first reviewed and signed by the production manager in charge of manufacturing the product. All products are reviewed by a quality assurance specialist who signs the certificate of analysis verifying that all appropriate quality systems were followed and that all specifications were met prior to shipment.
Shipping restrictions vary for specific countries and items. We recommend using a forwarding service for all shipments whose destination is not located in North America, Europe or Australia. This is mandatory if the shipment requires dry ice. We recommend using World Courier forwarding service; more information is available at www.worldcourier.com. Please inquire about your particular location.
Shipping costs vary depending on your location, as well as whether or not dry ice is required. All products delivered in solution will be shipped on dry ice. We ship via FedEx Priority Overnight or FedEx International Priority. Within the United States, standard shipping is approximately $15. International shipments range $40-$60. If your order requires dry ice or insurance, additional charges will apply. Please inquire for pricing specific to your location and order.
The simple answer is that it takes longer to make a high quality, modified oligonucleotides reproducibly. TriLink was formed in 1996 largely in response to the poor quality of modified oligonucleotides available from many vendors. We developed very methodical protocols geared to the manufacture of highly modified products. Our protocols require a great deal of attention to detail, as much of the work must be done manually to ensure success. Additionally, our purification methods are very time consuming, and we include 1-2 days for the analysis of products prior to shipping.
We ship via FedEx Priority which guarantees receipt by 10:30 am the following business day to most locations within the US. Our oligonucleotides are packaged in microcentrifuge tubes as lyophilized solids. Oligos are most stable lyophilized; however, we can ship them in solution in water or buffer upon request (an additional charge may apply). Unless requested, all oligos are shipped at room temperature. They are stable at room temperature, but should be stored at –20°C upon arrival. Compounds in solution, with a dimethoxytrityl group must be shipped on dry ice to ensure stability of the trityl group. Please note: dye labeled oligos are light sensitive. All oligos are shipped in an inner reflective Mylar envelope to shield them from light. This envelope can be used for storage.
Orders are prepared on a “first come, first served” principal. We do our best to accommodate your wishes by streamlining the process, however, our processes are generally firmly set, by both instrumentation and the training of the staff. Be aware that changing protocols may compromise the quality of the product. Most nights are already used for longer steps, such as deprotections, or dry-downs of larger samples. We can often schedule overtime if your requirements are truly urgent and you are willing to pay the added costs. This can save no more than a couple of days, since delivery is usually within 2 weeks. However, please discuss this option with us if you are interested.
No, not automatically. Universities are eligible for bulk and volume discounts as single organizations. If you have a number of buyers in your university, talk to your purchasing agent about obtaining an Annual Purchasing Agreement for the whole university.
You can get a discount by signing an Annual Purchase Agreement (APA). Based on your forecasted order volume, you will be eligible for a discount for the following twelve months. Some products are already heavily discounted and are excluded from the Annual Purchase Agreement, and custom compounds are quoted on a case-by-case basis at the best price currently possible. Bulk discounts are also available for many products. Call or email for details. You may also be interested in TriLink’s ResearchRewardsProgram, which twice annually awards grants of up to $10,000 in free products.
We will set up your account at the time of your initial order. You will need to provide a PO number or a credit card number and submit a credit application. Our accounting department will contact yours to obtain the remaining information required to establish credit. We retain the right to deny credit to any customer at any time.
NTPs are shipped the following business day, unless otherwise stated. Changes or additions may be made to your order until the time of shipping, which is 3pm PST. For oligonucleotide or custom compound orders, if we can pull the order out of production before synthesis is started, we can accommodate your request without incurring an extra fee. However, once synthesis of the product has begun, changes to the sequence or scale are no longer possible unless costs of the current synthesis are met. Please keep in mind that you may be responsible for the cost of any specialized reagents that were purchased to synthesize your compound, plus a 10% surcharge. PAGE or HPLC purification may be added anytime before an oligonucleotide is shipped.
Yes, click here for a list of distributors by region.
Yes. We offer full GMP quality material at all scales, both diagnostic grade as (FDA 21CFR 820) and pharmaceutical grade as (FDA 21CFR 210/211).
We can prepare any kind of nucleotide in our GMP facility. We first develop, if necessary, the process in our R&D lab, then validate it in our GMP facility with our equipment and reagents. Nearly all TriLink products are diagnostic grade GMP and manufactured in compliance with ISO 9001 and FDA 21CFR 820.
We are designing a new facility that will be suitable for the manufacturing of pharmaceutical grade materials by early 2014.
This is a decision for your regulatory department. However, we can offer advice based on your specific circumstances if you are unsure. Often, you do not need as stringent a manufacturing system as you may first believe.
Yes! TriLink adheres to the FDA 21CFR 820 requirements, as we believe this is the best way to manage the firm. We find the costs of managing separate documentation systems (one for research grade material and one for GMP grade material) are more than the cost savings associated with the reduced documentation required for research grade materials. Therefore, at TriLink there’s no such thing as research grade material. All compounds are manufactured under a GMP system at comparable prices to research grade material from our competitors.
Yes. We are ISO 9001 certified.
GMP stands for Good Manufacturing Practices, and refers to a system of manufacturing that guarantees reproducibility of product quality to set specifications. cGMP is simply Current Good Manufacturing Practices and refers to compliance with current regulations. It can be considered redundant since to be GMP compliant you must comply with current GMP regulations anyways. The requirements differ depending on what type of product is being manufactured and whether it is for pharmaceutical or diagnostic purposes. ISO stands for International Organization for Standardization, which offers a standard for operating a firm from management through manufacturing. It is more encompassing than GMP. QSR stands for Quality Systems Regulation, which are GMP standards described by the FDA for the manufacture of products for the diagnostic industry. The ISO and QSR systems each describe specific GMP standards. The ISO system pays more attention to the management of the firm and places a number of reporting loops in the firm to ensure attention to issues. The QSR system is more focused on the manufacturing systems, and the validation of those systems. Although neither standard is required to maintain GMP facilities, it is essential that a firm satisfies the requirements of the client’s quality system. Generally, this means conforming to ISO or QSR standards. Every product made at TriLink is manufactured under GMP. We are compliant with ISO 9001 and FDA 21CFR 820 regulations. We are in the process of developing a facility suited for the manufacture of pharmaceutical grade material for clinical trials.
We offer up to 10 grams per lot of most oligonucleotides and 100 grams per lot of modified nucleotides. Please inquire about our current capabilities.
We are located in San Diego, CA, in the Sorrento Mesa area just east of I-805 and just north of Miramar Marine Air Station. Our address is 9955 Mesa Rim Rd. San Diego, CA, 92121. Map It
We are open from 7:00 AM to 5:00 PM PST (10:00 AM to 8:00 PM EST).
Yes, if you order an oligonucleotide with multiple couplings of the same modified base your price will be adjusted accordingly after your order is placed. You will recieve a 25% discount on the second coupling and a 50% discount on all additional couplings of a particular base within a single oligonucleotide.
The Internal Modifications click menu works by inserting the modification at the current end of the oligonucleotide you are building. If you need to go back and insert an internal modification after you have filled in your entire sequence, you will either need to:
1. Delete back to the position of the modification, select it in the menu and fill in your remaining sequence, or
2. Select the modification, then carefully cut and paste the modification into the correct position.
Please always be sure to double check your sequence before placing your order.
CleanAmp™ Primers can be ordered here.
Several modifications are available in the amidite form and can be added to your sequence simply by clicking on them. These modifications are marked “Amidite.” For all other internal dyes and conjugates you will need to choose the appropriate selective placement linker. To add an internal dye or conjugate click the linker of choice, followed by the dye or conjugate of choice from the internal modifications menu. The modification will appear at the end of your current sequence. Continue adding bases as needed. To view all available selective placement linkers, dyes and other non-fluorescent conjugates, see our Custom Oligonucleotide Components menu.
TriLink offers numerous modifications that do not have standard list pricing and therefore do not appear in OligoBuilder®. These include 2’ Fluoro RNA bases, symmetrical branchers, cis-syn thymidine dimers, trimers and many more. Please email firstname.lastname@example.org for a quotation on any oligonucleotide modifications you do not see in OligoBuilder®. Be sure to include the sequence, backbone, modifications, starting synthesis scale or final yield and purification requirements.
Click here, then select your backbone to view expected yields for each.
Click here to learn about each purification option. If you are not sure which purification is most appropriate for your construct and application, select “Best Method” and our tech support team will assist you.
Click here to view backbone details.
Should I use the CleanAmp™ 7-deaza-dGTP Mix or just CleanAmp™ 7-deaza-dGTP alone?
For targets with <70% GC-content, addition of the CleanAmp™ 7-deaza-dGTP with standard dNTPs should amplify the target well. For targets with >70% GC-content, we recommend the CleanAmp™ 7-deaza-dGTP Mix for a robust amplification. Learn more about robust GC-rich target amplification in our white paper.
Have a question about CleanAmp™? Post a comment below.
I don’t see the modified nucleoside triphosphate that I need, can you make it for me?
Have a question about modified nucleoside triphosphates? Post a comment below.
How long does it take to activate CleanAmp™ dNTPs at 95°C and 60°C? – Jia Wang
Thank you for your interest in TriLink. CleanAmp™ dNTPs have a t 1/2 of 40 minutes at 95°C. We are currently developing a new, improved version of CleanAmp™ dNTPs that have a t 1/2 of 7 minutes. We are also working towards an isothermal solution… Read More.
Have a question about CleanAmp™ dNTPs? Post a comment below.
Wondering which RNA polymerase to use for incorporation of 2′ Fluoro and 2′ O-Methyl NTPs? We recommend T7 R&DNA™ and SP6 R&DNA™ polymerases from Epicentre. We’d like to hear from you – click the link below to leave your comment.
Collectively the TriLink Team has many years of experience in nucleic acid chemistry to offer. Post your question in the comment field below and we will get an answer to you as soon as we can.
Welcome to our new Ask An Expert blog. Answers to frequently asked questions are listed by topic on the Ask An Expert homepage. Please feel free to ask an expert by clicking on the comment link below. Thank you!