Hello, I would like to ask you if it is possible to use CleanTag Small RNA library prep kit also for piRNA sequencing or if the adapters bind only to miRNAs? -Best, Petra

Hello Petra,

CleanTag library preparation is a ligation based method that is designed to tag adapters onto small RNA species with a 3′ OH and 5′ P. piRNA is thought to have this type of structure and would therefore be compatible with the CleanTag kit.

Best Regards,

Joey

What’s the difference between the CleanTag™ Ligation Kit and CleanTag™ Library Prep Kit?

Both products included the reagents needed for the ligation of the 3′ adapter and 5 ‘ adapter. The CleanTag™ Small RNA Library Prep Kit (Cat# L-3206) also includes the reagents needed for the RT and PCR steps of library preparation which were not included in the CleanTag™ Ligation Kit (Cat# L-3203).

CleanTag™ Small RNA Library Prep Kit
(Cat# L-3206)
CleanTag™ Ligation Kit
(Cat# L-3203)
CleanTag™ 3′ Adapter CleanTag™ 3′ Adapter
CleanTag™ 5′ Adapter CleanTag™ 5′ Adapter
Enzymes, 1 & 2 Enzymes, 1 & 2
Buffers, 1 & 2 Buffers, 1 & 2
Reverse Transcriptase
RT Buffer
dNTP Mix (10 mM ea)
DTT
High Fidelity PCR Master Mix (2X)
RNase Inhibitor RNase Inhibitor

The CleanTag™ Ligation Kit was discontinued in early 2016.