I am currently using your ChromaLink™ Biotin Kit. I want to purify the labeled antibody that I have made by Protein G purification methods which will require the use of an elution buffer, namely 0.1 M Glycine at pH 2 or 3, which is quickly neutralized with 1 M Tris (pH 8 or 9). Do you know what the effect of the glycine is on the biotin linkage? Does glycine affect the chromophore?

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The bond that is formed between the antibody and the ChromaLink™ reagent is an amide bond, which is very stable in low pH. The ChromaLink™ chromaphore will not be affected by the low pH glycine buffer. Your protein G purification will not have any negative effect on the biotinylation of your protein.