I was wondering if the following modified nucleotides can be used by Taq polymerase in PCR. 1-Thio-2′-Deoxyadenosine-5′-Triphosphate (N-8001) 1-Thio-2′-Deoxycytidine-5′-Triphosphate (N-8002) – Chun-Nan Chen, Single Cell Technology

1-Thio-2′-Deoxyadenosine-5′-Triphosphate (N-8001) and 1-Thio-2′-Deoxycytidine-5′-Triphosphate (N-8002) can be incorporated by Taq in PCR. However, not all analogs will incorporate with the same efficiency in PCR; some optimization may be needed. We recommend performing some initial PCR experiments using natural:modified dNTPs in ratios such as 1:0, 3:1, 1:1, 1:3 and 0:1 to identify the best conditions for modified nucleotide incorporation with robust amplicon yield.