Do I need to remove Tris from my buffer prior to labeling my antibody?

The amines in a glycine or Tris-based buffer WILL interfere with optimal labeling of the antibody with HyNic, since HyNic reacts with amino groups. The All-in-One kit protocol buffer-exchanges your antibody into a phosphate-based buffer, free of amines, so the labeling reaction is optimized. The buffer exchange part of the protocol only takes a few minutes and is simple to perform.