DMSO improves PCR efficiency by disruption of GC-rich sequences. Typically, 0-10% DMSO is introduced into the reaction. DMSO can also inhibit PCR performance, so be sure that the percent DMSO introduced by CleanAmp™ Primers is not inhibitory to the reaction. At high DMSO percentages, the performance of CleanAmp™ Primers may be reduced. Therefore we recommend the addition of DMSO into your reaction only if absolutely necessary and to a maximum of 2% volume per volume.