How do I prepare a solution of my oligonucleotide with a predetermined molarity?

First determine the number of μmoles you have. Next, convert the units of your desired stock solution to M. For example, if you wish to prepare a 10 mM stock solution first convert your units to M by dividing by 1000 (resulting in 0.01 M in this example). Now simply divide the number of μmoles you have by the desired concentration of your final solution in M to determine the μL of buffer you will need to achieve that concentration. For example, if you have 0.2 μmoles of product and you wish to prepare a 10 mM stock solution, divide 10 mM first by 1000 to convert to 0.01 M, and then divide 0.2 μmoles by 0.01 M to obtain the number of μL needed, 20 μL in this case. This is a simplified, working version of the string of mathematical equations that underlie the procedure described. If you require more information, please contact us.