Are there protocols for polymorphic microsatellite analysis using IRDye labeled nucleotides instead of primers? There must be some issue because I cannot find any. -Janine

Dear Janine,

Thank you for your question. Labeled primers are typically used versus labeled dNTPs to allow for control over the number of fluorophores that are associated with each fragment. I am unaware of a protocol that uses labeled dNTPs.

If you decide to use labeled primers, TriLink offers a range of IRDyes for oligonucleotide labeling through OligoBuilder®. I’m sorry I could be of more help and good luck with your research.

Best regards,
Natasha Paul, PhD