What is the use of a Trimer Oligo Solution?

While randomers are often used in protein mutagenesis procedures, they have several short comings. First, it is very difficult to achieve a truly random mixture. The second and more problematic issue is the inevitable introduction of stop codons. Fortunately, the ability to direct combinatorial mutagenesis using randomized oligonucleotides has been advanced by the use of trimer oligonucleotides. Read More.