I was researching your CleanAmp primers and would like to know if they could be activated later in the PCR cycles. For example, I have 2 pairs of primers for PCR and would like the first pair to work for cycle 1 through 10 followed by a long activation period to have both pairs working. What’s the probability that the 2nd pair would be activated during the first 10 denaturing cycles. – Chun-Nan Chen

Thank you for your question about CleanAmp Primers. For your interest in having one primer pair work earlier in PCR, with the second primer pair being activated after the first 10 thermal cycles, the best way to achieve this is to prepare the first primer pair without modifications and to prepare the second primer pair with CleanAmp Precision modifications… Read More

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