Hi David,

Thank you for the inquiry. We can definitely assist you in creating a custom synthesis of this compound. Please contact our Technical Support team at sales@trilinkbiotech.com if you would like to discuss 6-Thio-2′-deoxyadenosine-5′-Triphosphate. We currently offer 6-thio-2′-deoxyguanosine-5′-triphosphate in our ready-made stocked catalog, but we do not have the adenosine analog stocked. Also, we are not familiar with an alternative commercial source for this material.

Best Regards,

Scott

We do provide fluorescently labeled nucleotides. Click Here for a list of our catalog dye-labeled nucleotides.

All of our modified nucleotides are submitted to quality control frequently to ensure that they still meet our specifications, therefore we do not assign expiration dates to our modified nucleotides other than our CleanAmp^TM dNTPs.

We can prepare any kind of nucleotide in our GMP facility. We first develop, if necessary, the process in our R&D lab, then validate it in our GMP facility with our equipment and reagents. All TriLink products are GMP and manufactured in compliance with ISO 9001.

The most common contaminants are the mono- and diphosphate forms of the nucleotide. Our purification procedures are designed to minimize the amount of other phosphates present, but since they result from hydrolysis that occurs at some finite rate with all nucleotides, they are generally present in the 0.5-3% range as detected by anion exchange HPLC. We routinely analyze older lots to identify these species and repurify the NTP if necessary to ensure it meets our purity specification (>90% for catalog products) before shipping. Two other potential contaminants are salts and inorganic phosphate. Salts are removed during the reverse phase HPLC and precipitation steps. Inorganic phosphate can be particularly problematic in some applications, therefore we take special measures to remove as much as possible. Every lot of NTP we manufacture is analyzed for the presence of inorganic phosphate using phosphorous NMR.

All of our nucleotides are prepared chemically using a variety of methods, several of which are proprietary to TriLink. We use a multi-step purification process that includes two DEAE columns and a reverse phase HPLC. Finally, the compounds are precipitated to remove any remaining salts and analyzed for quality. They are stored as an aqueous solution at -70°C.

Triphosphates of fluorescent nucleosides can be found in the Isomorphic-Intrinsically Fluorescent Analogs category. Their quantum yields and uses have been described in the literature. If you don’t see the triphosphate you need, request a quote.

Yes. Currently, we sell biotin and dabcyl labeled NTPs. Other labeled NTPs will be added to our product offering in the future.

Although NTPs are stable at room temperature for over a week, we ship them on ice to ensure quality in the event of a delay in transportation. We are still confident in the integrity of our NTPs if they have arrived thawed. However, if your ice has disappeared when your NTP arrives and you have concerns, please contact us immediately.

Most NTPs should be stable for several years when stored properly at -20^oC. We recommend aliquotting your NTP into multiple, single-use tubes upon first thawing to avoid repeated freeze/thaw cycles, which can lead to significant physical degradation. This is especially important for NTPs with amino modifications, which are less stable in solution than other NTPs.

Our triphosphates are resuspended in water so the pH of the solution will be very close to neutral.

Water.

The 1 μmole size of an NTP at a 10 mM concentration will have 100 μL of solution and a 100 mM concentration will have 10 μL (0.01 mL) of your NTP solution.

After purifying an NTP by anion exchange and reverse phase HPLC, the compound is precipitated and dissolved in water, an analytical scale anion exchange HPLC is run to verify each compound is >90% triphosphate. In addition, 31P NMR, Mass Spec and UV analyses are done to ensure the triphosphate has little or no inorganic phosphate. We then match the calculated molecular weight and confirm the concentration of the final solution.

Each NTP is accompanied by a certificate of analysis that lists the methods of analysis, concentration, purity, MW and extinction coefficient. A copy of the HPLC is also included.

A nucleoside is only the base and the sugar (5’-OH) while a nucleotide refers to a nucleoside with a phosphorylated sugar of any kind. This includes the phosphate groups on the 2’, 3’ or 5’ ends.