We are proud to introduce the latest addition to the CleanAmp™ Product Line, CleanAmp™ 7-deaza-dGTP for GC-rich target amplification. When DNA targets high in GC content are amplified, PCR product formation can often be compromised by inadequate strand separation and the propensity for complex secondary structure formation. The inability of the DNA polymerase to negotiate through regions of strong secondary structure, such as hairpins, often results in the formation of truncated PCR products. In addition, mis-priming amplification products are prevalent when primers are high in GC content. Despite the inherent challenges, detection of GC-rich sequences is becoming increasingly important for the molecular diagnosis of inherited diseases. Read More…