For the 3′ biotin TEG modification, is the biotin TEG directly off the 3′ end? Or is there an intervening phosphate, such as is pictured for the 5′ modification? If not, is it possible to have the 3′ end first phosphorylated and then modified with the biotin TEG? Thanks for your help! -Molly

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Dear Molly,

Thank you for your questions. The 3′ Biotin TEG modification does include a phosphate group between the TEG linker and the first nucleotide in your sequence. We look forward to helping you with your project!

Best regards,
Elizabeth