How can I clone my aptamer library? Is there a commercially available vector with convenient NdeI and SpeI sites that can be used to clone your aptamer libraries?

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The pSNAPf plasmid from New England Biolabs (cat no. N9183) has an NdeI site (nt 484) and SpeI site (nt 249) that can be used for cloning. Alternatively, a PCR fragment amplified with Taq polymerase will have TA ends that can be cloned into a TA cloning vector such as TOPO® TA vector from Life Technologies.