Should I still use additives such as DMSO, Betaine or Glycerol?

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CleanAmp™ 7-deaza-dGTP and CleanAmp™ 7-deaza-dGTP Mix function well on their own for most sequences with <80% GC-content. However, should you have a problematic target or one that is >80% GC-content, addition of any one of these additives may increase specificity. We have found 0.5M Betaine or 10% Glycerol give the best results.